Protein Disulphide Linkage Modeler
1. Define the protein sequence for the model.
Protein name
Sequence
2. Describe the chemistry used to generate sequence fragments.
Enzyme Trypsin Missed cleaves 0 1 2 3 4 5 6 Complete modifications Unmodified 4-vinyl-pyridine (Cys) Acrylamide (Cys) Iodoacetamide (Cys) Iodoacetic acid (Cys) Performic acid (Cys+O3) Performic acid (Met+O2) Performic acid (Met+O) Partial modifications Unmodified 4-vinyl-pyridine (Cys) Acrylamide (Cys) Iodoacetamide (Cys) Iodoacetic acid (Cys) Nitration (Y) Oxidation (M) Performic acid (Cys+O3) Performic acid (Met+O2) Performic acid (Met+O) Phosphorylation (S,T,Y) Phosphorylation (S,T) Phosphorylation (Y)
3. Enter the mass spectrum results for the model.
Average masses Monoisotopic masses Tolerance ± Tolerance ± Tolerance units Da  % ppm Mass type M  MH+
4. Filter the results for the model.
Max Free AAs 0 1 2 3 4 Max Fragments Linked 2 3 4 5 6 Max Link Attachments 1 2 3 4 5 6
5. Finally, to generate the models that fit this data set, Notes: To fill in this form with some example data, If you are using a browser that will not display xml Check here: